The table below provides a summary of the methods used to provide red blood cell results from the ADVIA hematology analyzer.
Result | Derivation | Details | Reported |
HCT (%) | Calculated | HCT = (MCVxRBC)÷10 | Routine hemogram, unless inaccurate (e.g. in vitro hemolysis) |
PCV (%) | Direct measurement | Centrifugation in a microhematocrit tube | Not on routine hemograms, unless RBC results do not reflect oxygen-carrying capacity (e.g. in vivo intravascular hemolysis) |
RBC count (million/μL) | Direct measurement | Events that pass through a laser beam | Routine hemogram, unless inaccurate (see HCT) |
Hemoglobin or Hgb (g/dL) | Direct measurement | RBC are lysed and hemoglobin is measured at a specific wavelength (540 nm) | Routine hemogram, except if lipemia affects results (false increase). Is not an accurate measure of oxygen-carrying capacity with in vivo intravascular hemolysis |
MCH (pg) | Calculated | (Hgb x 10)÷RBC | Routine hemogram, unless falsely increased (e.g. lipemia, agglutination, hemolysis) |
MCHC (g/dL) | Calculated from Hgb and HCT or PCV | (Hgb÷HCT or PCV) x 100 | see MCH |
Calculated or cellular hemoglobin (g/dL) | Calculated | (CHCM x HCT)÷1000 or (CHCM x RBC x MCV)÷1000 |
Not on routine hemograms, unless hemoglobin inaccurate (see above) |
CH (pg) | Direct measurement (= MCH in intact RBC only) | From internal complexity of intact RBC by side scattered (low angle) laser light | Not on routine hemograms, unless MCH inaccurate (see above) |
CHCM (g/dL) | Direct measurement (= MCHC in intact RBC only) | See CH | Not on routine hemograms, unless MCHC inaccurate (see above) |