On this page, we have provided links to videos that clinical pathology faculty and trainees at Cornell University have created for educational purposes.
In this video, we demonstrate how to measure a packed cell volume and total protein in EDTA-anticoagulated blood. The total protein result is provided on our hemogram results and is measured with a refractometer. The latter determines refractive index, which is influenced by soluble particles in plasma, such as protein and other so-called non-protein solids, e.g. urea nitrogen, glucose.
In this video, we demonstrate how to make a high-quality blood smear for blood smear examination.
This video demonstrates an optimal method for staining blood and cytologic smears with a rapid or quick stains, with examples of over- and understained blood smears.
A wealth of data is provided with the hemogram. Here, we outline how to approach this data to figure out what is going wrong with the animal, i.e. to identify pathologic processes such as anemia, inflammation or leukemia. We use hemogram results from a dog to show this approach, which involves breaking down the data into components for ease of interpretation: Erythrogram, leukogram and thrombogram, followed by plasma appearance and total protein measurement by refractometer.
A primer on leukemia
Leukemias, which are one of the hematopoietic neoplasms, are complex and can be difficult to diagnose or to distinguish into sub-types. Determining what type of leukemia an animal has is important because the type of leukemia impacts treatment and prognosis. In this first of a two part series of videos, we provide definitions and a classification scheme for leukemias, separating the different types as to stage of maturation (acute versus chronic) and origin (myeloid versus lymphoid) and introduce the concept of the primary acute and chronic leukemias versus a secondary leukemia associated with lymphoma (often thought of as “solid” hematopoietic neoplasms in extramedullary tissue versus blood and bone marrow with the primary leukemias). We call the latter “lymphoma with a leukemic phase”. Hemogram results are our clue as to the presence of the leukemia and the results that tip us off as to the presence of a leukemia in a given patient are briefly summarized.
Tools for leukemia diagnosis
In this second of the two part series, the tools that we use for phenotyping leukemias or differentiating reactive from neoplastic conditions involving lymphocytes are outlined. These tools are:
- Clonality: Differentiating reactive from neoplastic conditions involving lymphocytes
- Phenotyping with antibodies (immunophenotyping) or cytochemical stains): Used for classifying leukemias and identifying cell lineage.