Nov 012013
 

Photomicrographs of blood smear from a red-tail boa

 

Case information

A 5 year old male red-tail boa (boa constrictor) presented for weight loss. The snake had been completely inappetent for 6 to 7 months. The veterinarian submitted blood and feces to the Animal Health Diagnostic Center, associated with Cornell University, for a CBC and qualitative fecal exam (sugar flotation and zinc sulfate flotation). The fecal exam was negative. The most dramatic abnormality on the CBC was a marked lymphocytosis of 53.7 thousand/μl (reference interval 0.4-6.0 thousand/μL)1.

Evaluate the representative photomicrographs and consider the following:

  • What morphologic features can be used to distinguish heterophils from eosinophils and lymphocytes from thrombocytes in non-mammalian blood?
  • Identify the cells labeled A through F.
  • What morphologic abnormality is present in the lymphocytes?
  • Compare cells B and E. What is the significance of the cytoplasmic appearance of cell E?

Figure 1a: Peripheral blood smear (Wright's stain, 1000x)

Figure 1a: Peripheral blood smear (Wright’s stain, 1000x)

Figure 2a: Peripheral blood smear (Wright's stain, 1000x)

Figure 2a: Peripheral blood smear (Wright’s stain, 1000x)

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